Complete Amino Acid Sequence of a Polypeptide from Zea mays Similar to the Pathogenesis-Related-1 Family.

نویسندگان

  • J W Gillikin
  • W Burkhart
  • J S Graham
چکیده

A polypeptide serologically related to the tobacco pathogenesis-related-1 family of proteins has been purified from the root tissue of maize (Zea mays L.), and the complete amino acid sequence has been determined. The mature protein has a calculated molecular weight of 14,970 and isoelectric point of 4.2. The maize protein shows 66 to 68% amino acid identity with the tobacco pathogenesis-related-1 family and 55% identity with the tomato p14 protein.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification of a cDNA sequence coding for kruppellike factor 2b (Klf2b) from the skin mucosa of common carp (Cyprinus carpio)

Kruppel-like factors (Klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. UsingRT-PCR technique, a 456 bp cDNA fragment encoding N-terminus part of a Klf2b was isolated from the skinmucosa of common carp (Cyprinus carpio) using two degenerative oligonucleotid...

متن کامل

Molecular Genetics of the Maize ( Zea mays 1 . ) Aspartate Kinase - H omoserine Dehydrogenase Gene Family ’ Gary

Aspartate kinase (AK) and homoserine dehydrogenase (HSDH) are enzymes in the aspartate-derived amino acid biosynthetic pathway. Recent biochemical evidence indicates that an AK-HSDH bifunctional enzyme exists in maize (Zea mays 1.). In this report, we characterize three genes that encode subunits of AK-HSDH. Two cDNAs, pAKHSDHl and pAKHSDH2, containing the fullcoding sequence, and one partial c...

متن کامل

Cloning, Overexpression and in vitro Antifungal Activity of Zea Mays PR10 Protein

Background: Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities.Objective: The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens.M...

متن کامل

RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria.

A 5586 bp sequence (accession no. DQ278491), which includes the RNA polymerase II gene (RPB2) encoding the second largest protein subunit (RPB2), was obtained from the wheat biotype Phaeosphaeria nodorum (PN-w) by PCR amplification. The 3841 bp full length RPB2 gene contains two exons and a 52 bp intron, and encodes a complete 1262 amino acid protein. Similar to the C-terminals of the beta subu...

متن کامل

Nucleotide sequence of two potato alcohol dehydrogenase cDNAs.

We have isolated two cDNA clones for potato {Solatium tuberosum L. cv. Kennebec) alcohol dehydrogenase (ADH, EC. 1.1.1.1) from a tuber cDNA library (1). Each clone encodes a polypeptide of 380 amino acids that differ at only one position (amino acid 5). The nucleotide sequences show 96% identity These two sequences are highly similar with the sequence of another potato ADH cDNA clone (1) and th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 96 4  شماره 

صفحات  -

تاریخ انتشار 1991